Synthetic non-classical luminescence generation by enhanced silica nanophotonics based on nano-bio-FRET

SALINAS, CARINA; AMÉ, MARÍA VALERIA; BRACAMONTE, A. GUILLERMO

RSC Advances

Royal Society of Chemistry

Lugar: London; Año: 2020 vol. 10 p. 20620 - 20620

luorescent silica nanoparticles (NPs?(SiO2?Fluo)) were synthesized based on the classical Storber method for cyanobacteria labelling. Modified mono-coloured SiO2 NPs with fluorescein (Fl) and rhodamine B (RhB)were obtained (NPs?(SiO2?Fl) and NPs?(SiO2?RhB)). Moreover, multi-coloured SiO2 NPs, via the incorporation of both emitters (NPs?(SiO2?RhB?Fl)), were tuned for optimal emissions and the biodetection of cyanobacteria. NPs?(SiO2?Fl) and NPs?(SiO2?RhB?Fl) were optimized for detection via laser fluorescence microscopy and in-flow cytometry with laser excitation and fluorescence detection. By TEM, homogeneous SiO2 NPs of 180.0 nm in diameter were recorded. These sizes were slightly increased due to the covalent linking incorporation of fluorescent dye emitters to 210.0 nm with monocoloured fluorescent modified amine-organosilanes, and to 340.0 nm in diameter with multi-coloured dye incorporation. NPs?(SiO2?Fluo) showed variable emission depending on the dye emitter concentration, quan