Self-assembly dynamics and effect on synthetic nanobio-optical properties by hybrid monocolored silica nanoparticle labeling of Escherichia coli

GOMEZ PALACIOS, LUNA R.; SALINAS, CARINA; VEGLIA, ALICIA V.; AMÉ, MARIA VALERIA; BRACAMONTE, ANGEL GUILLERMO

JOURNAL OF NANOPHOTONICS

SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS

Año: 2022 vol. 16

1934-2608

he deposition of silica nanoparticles on Escherichia coli bacteria was investigated.The noncovalent interaction between the silanized surfaces and polar components of the biomembrane resulted in a nanobiostructure. This hybrid architecture showed stable conformationcharacteristics evaluated with different microscopy techniques, such as bright field confocalmicroscopy and transmission electron microscopy (TEM). Nanobioarchitectures were detectedwithin colloidal dispersions and in the absence of aqueous media. Nanobiointeractions wererelated to strong polar and hydrogen bridges’ noncovalent interactions. Thus, well-constitutedand defined nanobiostructures were observed by bright field confocal microscopy and TEM aftertheir preparation in optimal conditions. However, to evaluate their stability and internanobiostructure interactions, size distributions within variable periods were determined. Variable nanobioaggregate sizes were recorded according to nanoparticles and bacteria concentrat