Oxidative stress induced by ciprofloxacin in Staphylococcus aureus

BECERRA MARÍA CECILIA; ALBESA INÉS

Academic Press

Año: 2002 vol. 297 p. 1003 - 1003

p style="TEXT-ALIGN: justify; MARGIN: 0cm 0cm 0pt; mso-layout-grid-align: none" class="MsoNormal">Staphylococcus aureus with multiple sensitivity to ciprofloxacin, was investigated to detect alterations in the production of superoxide anion (O2-), other reactive oxidant species (ROS), and superoxide dismutase (SOD), and to relate them with ciprofloxacina accumulation and sensitivity. Oxidative stress was studied by means of Nitroblue Tetrazolium reaction (NBT) and chemiluminescence (CL); lucigenin was employed to detect O2-, and luminol was used to measure other ROS. Sensitive strains exhibited higher intracellular O2- increase than resistant ones when incubated with ciprofloxacin. SOD was determined in normal conditions and induction was investigated in the presence of ciprofloxacin. These assays demonstrated that resist