Leukotoxicity of pyoverdin, production of reactive oxygen species and effect of UV radiation

BECERRA, C.; ALBESA I.; ERASO, A

Academic Press

Lugar: Est; Año: 2001 vol. 285 p. 414 - 414

p style="TEXT-ALIGN: justify; MARGIN: 0cm 0cm 0pt; mso-layout-grid-align: none" class="MsoNormal">Pyoverdin was purified by solvent extraction, gel filtration, and ionic exchange chromatography. Assays of cytotoxic of pyoverdin were done with human leukocytes and macrophages from the peritoneum of mice. Both cell quantities showed a significant reduction. Death was followed by lysis in a dose-dependent form. The mechanism of action of pyoverdin involved the stimulation of reactive oxygen species (ROS) measured by Nitroblue Tetrazolium (NBT) reaction and chemiluminescence (CL). UV radiation at 368 nm increased the leukotoxicity; expositions of 5 min were enough to photostimulate the effect of pyoverdin on cellular oxidative metabolism, which increased between 35.4 and 53.2%. Genestein, an inhibitor of tyrosine kinases, counteracted the ROS stimuli of pyoverdin, suggesting endocytic mecha