:” Degradation of chlorobenzenes by a strain of Acidovorax avenae isolated from a polluted aquifer.”

MONFERRÁN, MAGDALENA V, ECHENIQUE JOSE R AND WUNDERLIN, DANIEL A

CHEMOSPHERE

ELSEVIER

Año: 2005 vol. 61 p. 98 - 98

0045-6535

bstractA subsurface microbial community was isolated from a polluted site of Suquı´a River (Córdoba—Argentina), acclimatedduring 15 days in aerobic conditions using 1,2-dichlorobenzene (1,2-DCB) as the sole carbon source. From this acclimated community, we isolated and identified by 16S rDNA analysis a strain of Acidovorax avenae, which was able to perform the complete biodegradation of 1,2-DCB in two days affording stoichiometric amounts of chloride. This pure strain was also tested for biodegradation of chlorobenzene (CB); 1,3-DCB and 1,4-DCB, giving similar resultsto the experiments using 1,2-DCB.The aromatic-ring-hydroxylating dioxygenase (ARHDO) a-subunit gene core, encoding the catalytic site of the large subunit of chlorobenzene dioxygenase, was detected by PCR amplification and confirmed by DNA sequencing. These results suggest that the isolated strain of A. avenae could use a catabolic pathway, via ARHDO system, leading to the formation of chloroc