Toxic, genotoxic, mutagenic, and bioaccumulative effects of metal mixture from settleable particulate matter on American bullfrog tadpoles (Lithobates catesbeianus)

DA COSTA, REGIANE LUIZA; SOUZA, IARA COSTA; MOROZESK, MARIANA; DE CARVALHO, LUANA BESERRA; CARVALHO, CLEONI DOS SANTOS; MONFERRÁN, MAGDALENA VICTORIA; WUNDERLIN, DANIEL ALBERTO; FERNANDES, MARISA NARCISO; MONTEIRO, DIANA AMARAL

ENVIRONMENTAL POLLUTION

ELSEVIER SCI LTD

Año: 2024 vol. 340

0269-7491

mphibians are more susceptible to environmental stressors than other vertebrates due to their semipermeable skin and physiological adaptations to living in very specific microhabitats. Therefore, the aim of the present study was to investigate the effects of a metal mixture from settleable particulate matter (SePM) released from metallurgical industries on Lithobates catesbeianus tadpoles. Endpoints analyzed included metal bioconcentration, morphological (biometrical indices), hematological parameters (hemoglobin and blood cell count), and erythrocyte DNA damage (genotoxicity and mutagenicity). American bullfrog tadpoles (Gosner´s stage 25) were kept under control condition (no contaminant addition) or exposed to a sub-lethal and environmentally relevant concentration (1 g.L−1) of SePM for 96 h. Tadpoles exposed to SePM exhibited elevated whole blood levels of Fe56, AL, Sn, Pb, Zn, Cr, Cu, Ti, Rb, V, Ce, La, Ag, As. SePM-exposed tadpoles showed a significant decrease in conditio