Dissecting thyroid hormone transport and metabolism in dendritic cells

GIGENA, NICOLAS; ALAMINO, VANINA A.; MONTESINOS, MM; NAZAR, M; LOUZADA, RUY A.; WAJNER, SIMONE M.; MAIA, ANA L.; MASINI-REPISO, ANA M.; CARVALHO, DENISE P.; CREMASCHI, GRACIELA A.; PELLIZAS, CLAUDIA G.

JOURNAL OF ENDOCRINOLOGY

BIOSCIENTIFICA LTD

Año: 2017 vol. 232 p. 337 - 337

0022-0795

e reported thyroid hormone (TH) receptor expression in murine dendritic cells (DCs) and 3,5,3´-triiodothyronine (T3)-dependent stimulation of DC maturation and ability to develop a Th1-type adaptive response. Moreover, an increased DC capacity to promote antigen-specific cytotoxic T-cell activity, exploited in a DC-based antitumor vaccination protocol, was revealed. However, putative effects of the main circulating TH, l-thyroxine (T4) and the mechanisms of TH transport and metabolism at DC level, crucial events for TH action at target cell level, were not known. Herein, we show that T4 did not reproduce those registered T3-dependent effects, finding that may reflect a homoeostatic control to prevent unspecific systemic activation of DCs. Besides, DCs express MCT10 and LAT2 TH transporters, and these cells mainly transport T3 with a favored involvement of MCT10 as its inhibition almost prevented T3 saturable uptake mechanism and reduced T3-induced IL-12 production. In turn, DCs expres