Thyroid hormone receptor alfa1-beta1 expression in epididymal epithelium from euthyroid and hypothyroid rats

DE PAUL AL; MUKDSI JH; PELLIZAS, CG; MONTESINOS, MM; GUTIÉRREZ S; DEL RÍO A; MALDONADO CA; TORRES AI

Springer

Año: 2008 vol. 129 p. 631 - 631

he objectives of the present work were to assess whether epithelial cells from the different segments of epididymis express TRalfa1–beta1 isoforms, to depict its subcellular immunolocalization and to evaluate changes in their expression in rats experimentally submitted to a hypothyroid state by injection of 131I. In euthyroid and hypothyroid groups, TR protein was expressed in epididymal epithelial cells, mainly in the cytoplasmic compartment while only a few one showed a staining in the nucleus as well. A similar TR immunostaining pattern was detected in the different segments of the epididymis. In hypothyroid rats, the number of TR-immunoreactive epithelial cells as well as the intensity of the cytoplasmic staining significantly increased in all sections analyzed. In consonance to the immunocytochemical analysis, the expression of TRalfa1– beta1 isoforms, assessed by Western blot revealed signi