Bacterial Lipopolysaccharide stimulates thyroglobulin gene expression at transcriptional level by involving TTF-1 and Pax8 transcription factors

VÉLEZ ML; COSTAMAGNA E; KIMURA ET; FOZZATTI L; PELLIZAS CG; MONTESINOS, MM; LUCERO AM; COLEONI AH; SANTISTEBAN P; MASINI-REPISO AM

Endocrine Society

Lugar: Chevy Chase, Maryland; Año: 2006 p. 3260 - 3260

he bacterial lipopolysaccharide (LPS) is a biological activator that induces expression of multiple genes in several cell types. LPS has been proposed as an etiopathogenic agent in autoimmune diseases. However, whether LPS affects the expression of autoantigens has not been explored. Thyroglobulin (TG) is a key protein in thyroid hormonogenesis and one of the major thyroid autoantigens. This study aimed to analyze the action of LPS on TG gene expression in Fisher rat thyroid cell line FRTL-5 thyroid cells. We demonstrate that LPS increases the TSH-induced TG protein and mRNA level. Evidence that the effect of LPS is exerted at the transcriptional level was obtained by transfecting the minimal TG promoter. The C element of the TG promoter, which contains sequences for paired box domain transcription factor 8 (Pax8) and thyroid transcription factor (TTF)-1 binding, is essential for full TG promoter