Triiodothyronine-stimulated dendritic cell vaccination boosts antitumor immunity against murine colon cancer

SOLER, MARÍA FLORENCIA; BRAVO-MIANA, ROCÍO DEL CARMEN; NEGRETTI-BORGA, DANA MARÍA; SUBIRADA, PAULA; ALAMINO, VANINA A.; SANCHEZ, MARÍA CECILIA; DONADIO, ANA CAROLINA; PELLIZAS, CLAUDIA GABRIELA; MONTESINOS, MARÍA DEL MAR

INTERNATIONAL IMMUNOPHARMACOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2022 vol. 110

1567-5769

iven the ability of dendritic cells (DCs) to modulate other immune players, their manipulation holds great potential for inducing efficient antitumor immunity. However, DC vaccine manufacturing deserve optimization since tumor cell cargo and DC functional state induced by maturation signals influence their in vivo immunogenic potential. We reported that triiodothyronine (T3) stimulates mice DCs’ maturation and their ability to promote pro-inflammatory and cytotoxic T-cell responses. This study aimed to evaluate the efficacy of T3-conditioned DC vaccination in a murine model of colon carcinoma, deciphering the molecular players involved; and to examine the effects of T3 on the maturation and activation of human DCs (huDCs). Bone marrow-derived DCs were exposed to T3 and MC38 cancer cells that underwent cell death (MC38-Apo/Nec) by UVB irradiation. Our results showed that MC38-Apo/Nec cells are efficiently uptaken by DCs and that T3 upregulates CD86 expression with increased production