Extracellular vesicles from thyroid cancer harbor a functional machinery involved in extracellular matrix remodeling

BRAVO-MIANA, ROCÍO DEL CARMEN; SOLER, MARÍA FLORENCIA; CESCHIN, DANILO GUILLERMO; ROYO, FÉLIX; NEGRETTI-BORGA, DANA MARÍA; AZKARGORTA, MIKEL; ELORTZA, FÉLIX; MONTESINOS, MARÍA DEL MAR; PELLIZAS, CLAUDIA GABRIELA; FALCÓN-PÉREZ, JUAN MANUEL; DONADIO, ANA CAROLINA

EUROPEAN JOURNAL OF CELL BIOLOGY

ELSEVIER GMBH

Año: 2022 vol. 101

0171-9335

xtracellular vesicles (EVs) participate in cell-stroma crosstalk within the tumor microenvironment and fibroblasts (Fb) contribute to tumor promotion in thyroid cancer. However, the role of tumor-stroma derived EVs still needs to be deciphered. We hypothesized that the interaction of thyroid tumor cells with Fb would liberate EVs with a specific proteomic profile, which would have an impact on EV-functionality in thyroid tumor progression-related events. Tumor (TPC-1, 8505c) and non-tumor (NThyOri) thyroid cells were co-cultured with human Fb. EVs, obtained by ultracentrifugation of conditioned media, were characterized by nanoparticle tracking analysis and western blotting. EV-proteomic analysis was performed by mass-spectrometry, and metalloproteinases (MMPs) were studied by zymography. EV-exchange was evaluated using immunofluorescence, confocal microscopy and FACS. EVs expressed classical exosome markers, with EVs from thyroid tumor cell-Fb co-cultures showing a proteomic profile