PRELIMINARY STUDY OF PRODUCTION AND IMMUNOMODULATORY ACTIVITY OF POLYSACCHARIDES DERIVED FROM WOOD DECAY FUNGI OF PATAGONIA

GALLO,ML; SOLER MF; MONTESINOS, MM; PELLIZAS CG; VELEZ, ML

Mar del Plata

Congreso; LXIII Reunión anual de la Sociedad de Investigación Clínica (SAIC) y LXVI Reunión anual de la Sociedad de Inmunología (SAI); 2018

Sociedad de Investigación Clínica (SAIC) y Sociedad Argentina de Inmunología (SAI)

Many species of fungi have medicinal properties. Among bioactive compounds of these organisms, polysaccharides (PS) can modulate the immune system. Several species from native forests of Patagonia may represent candidates to produce bioactive PS. Our aim was to evaluate PS production from Ganoderma australe, Inonotus crustosus and Laetiporus portentosus; and assess PS ability to activate mice dendritic cells (DCs), which drive adaptive immune responses. G.lucidum was used as reference species. Fungi were cultivated in different liquid culture media (AG, M1, M2) and their ability to produce extracellular PS (exo-PS) and PS from mycelium (structural-PS and basic-PS) was analyzed. Structural-PS production was higher than basic-PS and exo-PS production in all species. Ganoderma species were the best structural-PS producers. G.lucidum cultured in AG produced the highest amount of structural-PS (p<0.05), but regarding µg PS/mg mycelium, all species had a similar performance. In M2, G.australe produced the highest levels of structural-PS (p<0,0001) and showed the highest ratio µg PS/mg mycelium (p<0.001). In M1, G.australe and L.portentosus performed better than the rest of the species. Regarding PS production in different media, G.lucidum and I.crustosus showed the highest production, in total mass (p<0.005) and in µg PS/mg mycelium (p<0,0001) in AG, whereas in the other species the production was similar in all media. Considering that Ganoderma species produced the highest structural-PS levels, these fractions were tested in their ability to activate DCs. Mice bone marrow derived DCs were exposed to PS (10-300ng/ml) for 18-48h (activation control: lipopolysaccharide). Preliminary results indicated that PS from G.australe in M1 increased IL-12 production from DCs (vs basal) at 24h (p<0,05), CD86 at 18-24h (p<0,0001), and the expression of the maturation marker MHCII at 48h (p<0,0001), all parameters in a concentration-dependent manner. This is the first study reporting the potential ability of structural-PS from G.australe to activate DCs.