Interaction of amphiphilic cyclodextrins with phospholipids in Langmuir monolayers

JOHN JAIRO PINZON BARRANTES; RITA H.DE ROSSI; BRUNO MAGGIO; RAQUEL V. VICO

Carlos Paz

Congreso; XLII Reunión Anual de la Sociedad Argentina de Biofísica; 2013

Sociedad Argentina de Biofísica

Cyclodextrins (CDs) are water-soluble molecular cages with a hydrophobic interior that allows the inclusion and hence solubilization of various water-insoluble molecular species. These biocompatible cyclic oligosaccharides, with low toxicities and no immune response have also been used extensively in pharmaceutical applications as hydrophobic drug. CD-containing organized supramolecular structures can be obtained through self-assemblies of amphiphilic CDs1,2 or by their insertion into integrated systems such as lipid membranes.3 In previous work we study the capability of amphiphilic cyclodextrin to form Langmuir monolayer as well as the properties of these films.1,2 Here we present the properties of mixed films formed by an amphiphilic CD (CD16-betaCD) with palmitoyl oleyl phosphatidyl choline (POPC) at different mole fractions. The miscibility of two component been characterized by the surface pressure-area isotherms. The mixed isotherms lie in-between of the pure components. The interactions were examined by analyzing the mean molecular areas and quantified by the excess free energy. References 1Vico, R. Silva, O. Hoyos de Rossi, Rita, Maggio, B. Langmuir 2008. 7867. 2Vico, R. Hoyos de Rossi, Rita, Maggio, B. Langmuir 2010. 8407. 3Roux, M., Sternin, E., Bonnet, V., Fajolles,C., Djedaíni-Pilard, F. Langmuir 2013. 3677.