Engineering Pseudomonas fluorescens for biodegradation of 2,4-dinitrotoluene

MONTI MARIELA R.; SMANIA ANDREA M.; FABRO GEORGINA; ALVAREZ MARÍA E.; ARGARAÑA CARLOS E.

American Society for Microbiology

Lugar: Washington DC; Año: 2005 vol. 71 p. 8864 - 8864

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; mso-layout-grid-align: none">Using the genes encoding the 2,4-dinitrotoluene degradation pathway enzymes, the nonpathogenic psychrotolerantrhizobacterium Pseudomonas fluorescens ATCC 17400 was genetically modified for degradation of this priority pollutant. First, a recombinant strain designated MP was constructed by conjugative transfer from Burkholderia sp. strain DNT of the pJS1 megaplasmid, which contains the dnt genes for 2,4-dinitrotoluene degradation. This strain was able to grow on 2,4-dinitrotoluene as the sole source of carbon, nitrogen, and energy at levels equivalent to those of Burkholderia sp. strain DNT. Nevertheless, loss of the 2,4-dinitrotoluene degradative phenotype was observed for strains carrying pJS1. The introduction of dnt genes into the P. fluorescens ATCC 17400 chromosome, using a suicide chromosomal in