Nitosylation: an adverse factor in Hemolytic Uremic Syndrome. Antitoxin effect of Ziziphus mistol Griseb

AIASSA VIRGINIA, ALBRECHT CLAUDIA, BUSTOS PAMELA S., ORTEGA GABRIELA, ERASO ALBERTO J., ALBESA INÉS.

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2013 p. 381 - 381

oxins of Escherichia coli (STEC) causing Uremic Hemolytic Syndrome (UHS) generate oxidative stress in human blood with more production of nitric oxide (NO) than reactive oxygen species (ROS). Shiga toxin (Stx) together with the hemolysin (Hly) increased lipid oxidation, as evaluated by malondialdehyde MDA and oxidation of proteins. The addition of Ziziphus mistol Griseb extracts decreased NO, ROS, MDA and simultaneously caused an increase in the degradation of oxidized proteins to advanced oxidation protein products (AOPPs) in controls and samples with toxins. Furthermore, the nitrosylated proteins/AOPP ratio was reduced, due to the increase of AOPP. Z. mistol Griseb extracts exhibited a high proportion of polyphenols and flavonoids, with evident correlation with ferrous reduction antioxidant potential (FRAP). The plasma of eight children with UHS showed oxidative stress and NO stimulus, comparable to the effect of toxins during the assays in vitro. UHS children presented hig