Rapid and effective photodynamic treatment of biofilm infections using low doses of amoxicillin-coated gold nanoparticles

ROCCA, DIAMELA M.; SILVERO C., M. JAZMIN; AIASSA, VIRGINIA; CECILIA BECERRA, M.

Photodiagnosis Photodynamic Therapy

ELSEVIER SCIENCE BV

Año: 2020 vol. 31

1572-1000

acterial biofilm are complex microbial communities covered by a matrix of extracellular polymeric substances,which develops when a community of microorganisms irreversibly adheres to a living or inert surface. Thisstructure is considered an important virulence factor because it is difficult to eradicate and often responsible for treatment failures. This adherent community represents one of the greatest problems in public health due to thecontinued emergence of conventional antibiotic-therapy resistance. Photodynamic Antimicrobial Therapy (PACT) is a therapeutic alternative and promises to be an effective treatment against multiresistant bacteria biofilm, demonstrating a broad spectrum of action. This work demonstrates the reduction in biofilms of relevant clinical isolates (as Pseudomonas aeruginosa and Staphylococcus aureus) treated with PACT using low concentrations of amoxicillin-coated gold nanoparticles (amoxi@AuNP) as a photosensitizer. Moreover, the viabilityreduction of 60% in