Artículos
Título:
Assembly of Glucose Oxidase and Different Polyelectrolytes by Means of Electrostatic Layer-by-Layer Adsorption. Analytical Applications.
Autor/es:
M. C. RODRÍGUEZ AND G. A. RIVAS*
Referencias:
Año: 2004 vol. 16 p. 1717 - 1717
Resumen:
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Abstract
A rational strategy for the construction of a bioelectrocatalytic architecture by means of alternate electrostatic
adsorption is described. Multilayer films containing glucose oxidase (GOx) and different polyelectrolytes were
assembled onto a thiolated-gold surface and the resulting bioelectrode was used for glucose biosensing. The
supramolecular multistructure was prepared by assembling polyethylenimine and Nafion (as anti-interference barrier),
followed by the adsorption of polyethylenimine and DNA (as stabilizing film) and finally by the alternate deposition
of polyethylenimine and glucose oxidase (as a biocatalytic layer). The influence of the deposition time and
concentration of polyelectrolytes, organization and number of layers on the sensitivi