"Enzymatic biosensor for the electrochemical detection of 2,4-dinitrotoluene biodegradation derivatives"

M. C. RODRÍGUEZ, M. MONTI, C. ARGARAÑA AND G. A. RIVAS*

TALANTA

elsevier

Año: 2005

0039-9140

font face="Times New Roman" size="1"> In this work, we demonstrate for the first time that 4-methyl-5-nitrocatechol (4M5NC) and 2,4,5-trihydroxytoluene (2,4,5-THT), two compoundsobtained from the 2,4-DNT biodegradation are recognized by polyphenol oxidase as substrates.Anamperometric biosensor is described for detectingthese compounds and for evaluating the efficiency of the 2,4-DNT conversion into 4M5NC in the presence of bacteria able to produce the 2,4-DNT-biotransformation. The biosensor format involves the immobilization of polyphenol oxidase into a composite matrix made of glassy carbon microspheres and mineral oil. The biosensor demonstrated to be highly sensitive for the quantification of 4M5NC and 2,4,5-THT. The analytical parameters for 4M5NC are the following: sensitivity of (7.5±0.1)×10