Adsorption and Electrooxidation of Nucleic Acids at Glassy Carbon Electrodes Modified with Multiwalled Carbon Nanotubes Dispersed In Polylysine

YAMILE JALIT; MONICA MORENO; FABIANA GUTIÉRREZ; A. SÁNCHEZ ARRIBAS F; MANUEL CHICHARRO; ESPERANZA BERMEJO; ANTONIO ZAPARDIEL; CONCEPCION PARRADO; GUSTAVO RIVAS; MARCELA RODRÍGUEZ

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2013 vol. 25 p. 1116 - 1116

e report the advantages of the adsorption and electrooxidation of oligonucleotides and calf-thymus double strand- ed DNA (dsDNA) at glassy carbon electrodes (GCE) modified with a dispersion of multiwalled carbon nanotubes (MWCNT) in poly-l-lysine (Plys) (GCE/MWCNT-Plys). Important enhancement in the guanine oxidation signal was obtained by adsorptive stripping voltammetry (AdSV) due to a most favorable interaction between the nega- tively charged DNAs and the positively charged Plys that support the MWCNT. The layer of oligo(dG)11 immobi- lized at GCE/MWCNT-Plys was successfully used for the selective detection of the hybridization event using oli- go(dG)11/oligo(dC)11 as model.