Adsorption and Electrooxidation of DNA at Glassy Carbon Electrodes Modified with Multiwall Carbon Nanotubes Dispersed in Glucose Oxidase

FABIANA A. GUTIÉRREZ; M. DOLORES RUBIANES; GUSTAVO A. RIVAS

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2013 vol. 25 p. 1135 - 1135

e are proposing for the first time the successful immobilization of DNA at glassy carbon electrodes (GCE) modi- fied with carbon nanotubes (CNT) dispersed in glucose oxidase (GOx) (GCE/CNT-GOx) either by direct adsorp- tion or by layer-by-layer self-assembling using polydiallyldimethylamine (PDDA). The presence of GOx allows an efficient dispersion of CNT and gives a most favorable environment that promotes the adsorption and makes possi- ble a more sensitive electrooxidation of DNA. The PDDA incorporated in the self-assembled architecture largely facilitates the adsorption and electrooxidation of dsDNA and the adsorbed layer can be successfully used for evalu- ating the interaction of DNA with methylene blue.