Assembly of Glucose Oxidase and Different Polyelectrolytes by Means of Electrostatic Layer-by-Layer Adsorption. Analytical Applications.

M. C. RODRÍGUEZ AND G. A. RIVAS*

ELECTROANALYSIS

VCH

Año: 2004 vol. 16 p. 1717 - 1717

1040-0397

font face="AdvTTB" size="1"> Abstract A rational strategy for the construction of a bioelectrocatalytic architecture by means of alternate electrostatic adsorption is described. Multilayer films containing glucose oxidase (GOx) and different polyelectrolytes were assembled onto a thiolated-gold surface and the resulting bioelectrode was used for glucose biosensing. The supramolecular multistructure was prepared by assembling polyethylenimine and Nafion (as anti-interference barrier), followed by the adsorption of polyethylenimine and DNA (as stabilizing film) and finally by the alternate deposition of polyethylenimine and glucose oxidase (as a biocatalytic layer). The influence of the deposition time and concentration of polyelectrolytes, organization and number of layers on the sensitivi