Glucose Biosensor Based on the Layer-by-layer Self Assembling of Glucose Oxidase and Chitosan Derivatives on a Thiolated Gold Surface

S. A. MISCORIA; JACQUES DESBRIERES; PIERRE LABBÉ; G. D. BARRERA; G. A. RIVAS

ANALYTICA CHIMICA ACTA

Elsevier

Año: 2006 vol. 578 p. 137 - 137

b> Abstract The work proposed here deals with the design and characterization of biorecognition layers for the amperometric glucose determination based on the self-assembling of new chitosan derivatives, Nafion and glucose oxidase onto thiolated gold electrodes. The supramolecular multistructure is obtained by deposition of a layer of chitosan derivative (quaternized or hydrophobic) onto the gold surface modified with the sodium salt of 3-mercapto-1-propansulfonic acid, followed by the deposition of a layer of Nafion (as anti-interference barrier) and by the alternate deposition of the chitosan derivative and glucose oxidase (as biocatalytic layer). The influence of the deposition time and concentration of polyelectrolytes, organization and number of layers, and nature of the chitosan derivative on the sensitivity and selectivity of the bioelectrode is examined and optim