Dispersion of multi-wall carbon nanotubes in glucose oxidase: Characterization and analytical applications for glucose biosensing

FABIANA GUTIÉRREZ; MARÍA D. RUBIANES; GUSTAVO A. RIVAS

ELSEVIER SCIENCE SA

Lugar: Amsterdam; Año: 2012 vol. 161 p. 191 - 191

font size="1" face="GulliverRM"> In this work we report for the first time the use of the enzyme glucose oxidase (GOx) to efficiently disperse multiwall carbon nanotubes (CNT) and to confer biorecognition properties to the dispersed nanotubes. The optimum dispersion was obtained by sonicating for 15 min 1.0 mg/mL CNT in 1.0 mg/mL GOx solution prepared in 50:50 ethanol/water. The dispersion was evaluated by Scanning Electron Microscopy (SEM), Infrared (FT-IR) and Ultraviolet?visible (UV?vis) Spectroscopy. The electrochemical characterization of glassy carbon electrodes (GCE) modified with the dispersion (by dropping) was performed by Electrochemical Impedance Spectroscopy (EIS), Cyclic Voltammetry (CV), and Amperometry. The amount of electroactive GOx deposited on GCE (GCE/CNT?GOx) was 1.02 ??