Impedimetric and amperometric genosensors for the highly sensitive quantification of SARS-CoV-2 nucleic acid using an avidin-functionalized multi-walled carbon nanotubes biocapture platform

MUJICA, MICHAEL LÓPEZ; TAMBORELLI, ALEJANDRO; CASTELLARO, ANDRÉS; BARCUDI, DANILO; RUBIANES, MARÍA D.; RODRÍGUEZ, MARCELA C.; SAKA, HÉCTOR A.; BOCCO, JOSÉ L.; DALMASSO, PABLO R.; RIVAS, GUSTAVO A.

Biosensors and Bioelectronics: X

Elsevier

Año: 2022 vol. 12

2590-1370

e report two novel genosensors for the quantification of SARS-CoV-2 nucleic acid using glassy carbon elec-trodes modified with a biocapture nanoplatform made of multi-walled carbon nanotubes (MWCNTs) non-covalently functionalized with avidin (Av) as a support of the biotinylated-DNA probes. One of the geno-sensors was based on impedimetric transduction offering a non-labelled and non-amplified detection of SARS-CoV-2 nucleic acid through the increment of [Fe(CN)6]3-/4- charge transfer resistance. This biosensor pre-sented an excellent analytical performance, with a linear range of 1.0 × 10