BIOFUNCTIONALIZED CARBON NANOSTRUCTURES-BASED BIOSENSORS: NEW ANAYTICAL TOOLS FOR THE QUANTIFICATION OF BIOMARKERS

RIVAS, GUSTAVO A.

Conferencia; XXIV International Symposium on Advances in Extraction Technologies; 2022

This presentation is focused on the design, characterization and analytical applications of nanobiosensors based on the use of rationally functionalized carbon nanotubes and graphenaceous materials. Special attention is given to the influence of the functionalization scheme and the selection of biorecognition/transduction events on the analytical performance of the resulting nanoarchitectures for the quantification of relevant biomarkers.The electrochemical biosensors were obtained by modification of glassy carbon-, screen printed carbon- or gold-electrodes with multi-walled carbon nanotubes (MWCNTs) functionalized with biomolecules or graphenaceous materials modified with polymers as platforms for the selective anchoring of the biorecognition elements. We will discuss the advantages of using MWCNTs non-covalently functionalized with avidin in two directions, for the development of hydrogen peroxide and glucose enzymatic biosensors, and for the construction of genosensors devoted to the detection of the breast cancer biomarker BRCA1 and SARS-CoV-2 nucleic acid, at femtomolar and atomolar levels, respectively. The development of highly sensitive and selective nanobiosensors for the quantification of the important cancer biomarker microRNA-21 will be also presented. These genosensors were based on the use of graphene oxide self-assembled with polydiallyldimethylamine or reduced graphene oxide modified with chitosan as platforms to immobilize the DNA probe in connection with plasmonic and impedimetric transduction, respectivelyIn summary, the critical selection of the nanomaterial (bio)functionalization route and the transduction strategy are key aspects for the development of highly sensitive and selective biosensors.