ACOSTA RODRÍGUEZ EVA VIRGINIA
Congresos y reuniones científicas
Título:
B cells regulate the inflammatory CD4+ T cell response in Trypanosoma cruzi infection.
Autor/es:
GOROSITO SERRÁN M; TOSELLO BOARI J; RAMELLO MC; BECCARIA CG; FIOCCA VERNENGO F; BERMEJO DA; AMEZCUA VESELY MC; MONTES CL; ACOSTA RODRIGUEZ EV; GRUPPI A
Reunión:
Congreso; LVII Reunión Anual de la Sociedad Argentina de Inmunología; 2014
Resumen:
B cell-deficient mice (mMT) infected with T. cruzi are
able to control parasitemia similar to C57BL/6 wild type
(WT) mice but present increased parasitemia by 10 days
post-infection (dpi) and accelerated mortality. mMT and
WT mice have similar tissue parasite load as determined
by RT-PCR, indicating that mortality is not caused by
uncontrolled parasitism. To assess possible causes of
the increased susceptibility of mMT mice, we analyzed
cytokine production and CD4+T cell responses in mMT
and WT mice infected i.p. with 10000 trypomastigotes
Y-br strain. Groups of 4-7 mice were sacrificed by 4, 9,
15, 20 dpi and sera and splenocytes were obtained. By 9
dpi, amounts of serum IFNg were increased in both infected
mice strains, but were higher in mMT mice (p<0.01).
Serum TNF amounts were increased in both mice strains
by 9 dpi and afterwards diminished in WT and increased
in mMT mice (15 and 21 dpi, p<0.001). CD4+T cells where
the main IFNg producing population by 9 dpi and were
higher in mMT mice in comparison to WT (p=0.0013).
CD4+T cells were also the main TNF producers by 15
and 20 dpi and in mMT mice preferentially had a proinflammatory
phenotype (CD4+Ly6C+). IL-10-producing
cells were reduced in infected mMT mice due to not only
the absence of IL-10+B cells present in WT mice, but also
a decrease in the numbers of IL10-producing CD4+T cells
(15 dpi, p<0.001). In vitro co-culture experiments showed
that B cells controlled TNF production in splenocytes
from infected mice incubated with T. cruzi antigens. TNF
neutralization by mAbs prevented the cachexia observed
in infected mMT mice but accelerated even more their
mortality due to an exacerbated Th1 response with high
amounts of IL12, IFNg, TNF. In these mice, CD4+Ly6C+
cells were increased in spleen at 30 dpi. Our data suggests
that B-cell absence in T. cruzi infection could cause an
altered activation of the CD4+T-cells, leading to a systemic
imbalance in the host and mortality.