Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents

QUINTEROS MA; AIASSA MARTINEZ I; DALMASSO PR; PAEZ PL

International Journal of Biomaterials

Hindawi Publishing Corporation

Año: 2016 vol. 2016 p. 1 - 1

urrently, the biosynthesis of silver-based nanomaterials attracts enormous attention owing to the documented antimicrobialproperties of these ones. This study reports the extracellular biosynthesis of silver nanoparticles (Ag-NPs) using a Pseudomonasaeruginosa strain from a reference culture collection. A greenish culture supernatant of P. aeruginosa incubated at 37∘C with a silvernitrate solution for 24 h changed to a yellowish brown color, indicating the formation of Ag-NPs, which was confirmed by UVvisspectroscopy, transmission electron microscopy, and X-ray diffraction. TEM analysis showed spherical and pseudosphericalnanoparticles with a distributed size mainly between 25 and 45 nm, and the XRD pattern revealed the crystalline nature of AgNPs.Also it provides an evaluation of the antimicrobial activity of the biosynthesized Ag-NPs against human pathogenic andopportunistic microorganisms, namely, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Prote