Naphthoquinones inhibit formation and viability of Yersinia enterocolitica biofilm

DI MARCO, NATALIA I.; PÁEZ, PAULINA L.; LUCERO-ESTRADA, CECILIA S. M.; PUNGITORE, CARLOS R.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

SPRINGER

Año: 2021 vol. 37

0959-3993

bstract: The capacity of different naphthoquinones to inhibit and eradicate Yersinia enterocolitica biofilm was investigated and possible mechanisms of action were evaluated. Inhibition of biofilm formation and cell viability, quorum sensing (QS) inhibition and oxidative stress generation of 23 naphthoquinones were assayed against Yersinia enterocolitica. The best anti-biofilm agents at 100 µmol l−1 were compounds 3, 11 and 13, which showed biofilm inhibition higher than 75%. Compound 3 was the most effective against biofilm forming capacity of Y. enterocolitica WAP 314 with a minimum biofilm inhibitory concentration (MBIC) of 25 µmol l−1; while against Y. enterocolitica CLC001, the lowest MBIC was 6.1 µmol l−1 for compound 11. Acyl-homoserine lactones production was decreased with compound 13. We showed that the oxidative stress influence biofilm growth, by means of ROS and RNI increment. All treatments increased ROS and RNI values in the biofilm of both strains; wh