Silver bionanoparticles toxicity in trophoblast is mediated by nitric oxide and glutathione pathways

BUSTOS, PAMELA SOLEDAD; QUINTEROS, MELISA DE LOS ÁNGELES; GOMEZ, DIEGO SEBASTIAN; ORTEGA, MARÍA GABRIELA; PÁEZ, PAULINA LAURA; GUIÑAZÚ, NATALIA LORENA

TOXICOLOGY

ELSEVIER IRELAND LTD

Año: 2021 vol. 454

0300-483X

ilver bionanoparticles (AgNPs) biosynthesized by Pseudomonas aeruginosa culture supernatant have an important antibacterial activity mediated by ROS increase; however their toxicity in human cells is not known. Due to the high susceptibility of the developing tissues to xenobiotics, the aim of this study was to investigate the AgNPs effect on first trimester human trophoblasts. The HTR8/SVneo cell line was treated with AgNPs (0.3?1.5 pM), for 6 and 24 h. Cell viability, reactive nitrogen and oxygen species (RNS and ROS) production, nitric oxide synthase expression, antioxidant defenses and biomolecule damage were evaluated. The exposure to AgNPs produced changes in HTR8/SVneo cell morphology and decreased cell viability. Alterations in redox balance were observed, with an increase in ROS and RNS levels, and NOS2 protein expression. Superoxide dismutase and catalase augmented their activity accompanied with a decreased in glutathione content and glutathione S-transferase activity. Prot