Synergic activity of oligostyrylbenzenes with amphotericin B against Candida tropicalis biofilms

QUINTEROS, MELISA A.; TOLOSA, JUAN; GARCÍA?MARTÍNEZ, JOAQUÍN C.; PÁEZ, PAULINA L.; PARAJE, MARÍA G.

YEAST

JOHN WILEY & SONS LTD

Año: 2021

0749-503X

ntimicrobial drug resistance is a serious challenge in clinical settings worldwide, withbiofilm formation having been associated with this problem. In the present study, thesynergism of oligostyrylbenzene (OSB) compounds in combination with amphotericinB (AmB) against Candida tropicalis biofilms was investigated. In addition, the toxicityin human blood cells was determined. Synergistic combinations of OSBs and AmBwere evaluated to consider future effects of OSBs in vivo. The checkerboard microdilutionmethod was used to study the interactions of one anionic (1) and two cationic(2 and 3) OSBs with AmB. We investigated the effects of OSBs on reactiveoxygen species (ROS) and the levels of the reactive nitrogen intermediates (RNIs).The cellular stress affected biofilm growth through an accumulation of ROS and RNI,at synergistic concentrations of OSBs and AmB. Furthermore, significant surfacetopography differences were noted upon treatment with the OSB 2/AmB combination,using confocal laser