Novel antifungal activity of oligostyrylbenzenes compounds on Candida tropicalis biofilms

QUINTEROS, MELISA A; GALERA, IVANA L. D; TOLOSA, JUAN; GARCÍA-MARTÍNEZ, JOAQUÍN C; PÁEZ, PAULINA L; PARAJE, MARÍA G

MEDICAL MYCOLOGY

TAYLOR & FRANCIS LTD

Año: 2021 vol. 59 p. 244 - 244

1369-3786

s sessile cells of fungal biofilms are at least 500-fold more resistant to antifungal drugs than their planktonic counterparts, there is a requirement for new antifungal agents. Olygostyrylbenzenes (OSBs) are the first generation of poly(phenylene)vinylene dendrimers with a gram-positive antibacterial activity. Thus, this study aimed to investigate the antifungal activity of four OSBs (1, 2, 3, and 4) on planktonic cells and biofilms of Candida tropicalis. The minimum inhibitory concentration (MIC) for the planktonic population and the sessile minimum inhibitory concentrations (SMIC) were determined. Biofilm eradication was studied by crystal violet stain and light microscopy (LM), and confocal laser scanning microscopy (CLSM) was also utilized in conjunction with the image analysis software COMSTAT. Although all the OSBs studied had antifungal activity, the cationic OSBs were more effective than the anionic ones. A significant reduction of biofilms was observed at MIC and supraMIC50