Arabidopsis proline dehydrogenase contributes to flagellin mediated PAMP-triggered immunity by affecting RBOHD

GEORGINA FABRO; YANINA SOLEDAD RIZZI; MARIA ELENA ALVAREZ

MOLECULAR PLANT-MICROBE INTERACTIONS

AMER PHYTOPATHOLOGICAL SOC

Año: 2016

0894-0282

lants activate different defense systems to counteract the attack of microbial pathogens. Among them, the recognition of conserved microbial/pathogen associated molecular patterns (MAMPs/PAMPs) by pattern-recognition receptors stimulates MAMP/PAMP-triggered immunity (MTI/PTI). In recent years, the elicitors, receptors and signaling pathways leading to PTI have been extensively studied. However, the contribution of organelles to this program deserves further characterization. Here, we studied how processes altering the mitochondrial electron transport chain (mETC) influence PTI establishment. With particular emphasis we evaluated the effect of proline (Pro) dehydrogenase (ProDH), an enzyme that can load electrons into the mETC and regulate the cellular redox state. We found that mETC uncouplers (antimycin/rotenone) and MnSOD deficiency impair flg22-induced responses, such as accumulation of reactive oxygen species (ROS) and bacterial growth limitation. ProDH mutants also reduce these d