Subcellular localization of the Hpa RxLR effector repertoire Identifies a tonoplast-associated protein HaRxL17 that confers enhanced plant susceptibility.

JONATHAN D. G. JONES

PLANT JOURNAL

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Malden; Año: 2012 vol. 69 p. 252 - 252

0960-7412

ilamentous phytopathogens form sophisticated intracellular feeding structures called haustoria in plant cells.Pathogen effectors are likely to play a role in the establishment and maintenance of haustoria in addition totheir better-characterized role in suppressing plant defence. However, the specific mechanisms by whichthese effectors promote virulence remain unclear. To address this question, we examined changes insubcellular architecture using live-cell imaging during the compatible interaction between the oomyceteHyaloperonospora arabidopsidis (Hpa) and its host Arabidopsis. We monitored host-cell restructuring ofsubcellular compartments within plant mesophyll cells during haustoria ontogenesis. Live-cell imaginghighlighted rearrangements in plant cell membranes upon infection, in particular to the tonoplast, which waslocated close to the extra-haustorial membrane surrounding the haustorium. We also investigated thesubcellular