FABRO GEORGINA
Congresos y reuniones científicas
Título:
Functional analysis of plant-pathogenic oomycete effectors and their targets inside plant cell (POETIC)
Autor/es:
MARIE-CECILE CAILLAUD; SOPHIE J. M. PIQUEREZ; FABRO GEORGINA; NAVEED ISHAQUE; SUE DONOVAN; BEYNON, J.; JONATHAN D. G. JONES
Lugar:
Praga
Reunión:
Congreso; Society for Experimental Biology Main Meeting, Prague 2010; 2010
Institución organizadora:
Society for Experimental Biology
Resumen:
Plant-associated organisms secrete molecules
to attenuate plant defence signalling and enable
colonization of plant tissue. Like fungal rust and powdery
mildew pathogens, oomycetes form specialized
feeding structures “haustoria”. Pathogen effectors are
translocated via haustoria into host cells for haustorialestablishment and maintenance, to immunocompromise
the host and for establishing the infection site as a
metabolic sink. The effectors are presumed to be first
secreted from the pathogen, and then taken up by the
host cell, perhaps by endocytosis, via an N-terminal
RxLR uptake motif. Hitherto, in planta subcellular
localisation of effectors translocated from oomycetes
into the plant cell was not achieved, though this was
achieved for bean rust. As part of a contribution to an
ERA-PG project coordinated by Jim Beynon, the Jones
laboratory tested candidate effector genes mined from
the genome sequence of Arabidopsis downy mildew
(Hyaloperonospora arabidopsidis -Hpa) using P. syringae
via type III secretion. They identified ~65 effectors
that confer enhanced/decreased virulence of Pst on
at least one Arabidopsis accession, and 35 have been
prioritized. We aim to analyse in planta, the modes of
action of promising RxLR effectors from Hpa that confer
enhanced/decreased virulence. The main objectives are
the subcellular localisation of Hpa effectors in the host
cell and the identification of Hpa effector plant targets.
The characterization of oomycete effectors that enter the
plant cell, their functions and protein-uptake mechanisms
will be a fascinating area for understanding the basis of
plant pathogenesis and will shed light on fundamental
cellular mechanisms in plants.
to attenuate plant defence signalling and enable
colonization of plant tissue. Like fungal rust and powdery
mildew pathogens, oomycetes form specialized
feeding structures “haustoria”. Pathogen effectors are
translocated via haustoria into host cells for haustorialestablishment and maintenance, to immunocompromise
the host and for establishing the infection site as a
metabolic sink. The effectors are presumed to be first
secreted from the pathogen, and then taken up by the
host cell, perhaps by endocytosis, via an N-terminal
RxLR uptake motif. Hitherto, in planta subcellular
localisation of effectors translocated from oomycetes
into the plant cell was not achieved, though this was
achieved for bean rust. As part of a contribution to an
ERA-PG project coordinated by Jim Beynon, the Jones
laboratory tested candidate effector genes mined from
the genome sequence of Arabidopsis downy mildew
(Hyaloperonospora arabidopsidis -Hpa) using P. syringae
via type III secretion. They identified ~65 effectors
that confer enhanced/decreased virulence of Pst on
at least one Arabidopsis accession, and 35 have been
prioritized. We aim to analyse in planta, the modes of
action of promising RxLR effectors from Hpa that confer
enhanced/decreased virulence. The main objectives are
the subcellular localisation of Hpa effectors in the host
cell and the identification of Hpa effector plant targets.
The characterization of oomycete effectors that enter the
plant cell, their functions and protein-uptake mechanisms
will be a fascinating area for understanding the basis of
plant pathogenesis and will shed light on fundamental
cellular mechanisms in plants.
