Elucidating the role of Hyaloperonospora arabidopsidis RxLR effector proteins on the suppression of the plant immune system

JENS STEINBRENNER; FABRO GEORGINA; SUE DONOVAN; SHAHID MUKTHAR; BEYNON, J.; JONATHAN D. G. JONES

Pacific Grove

Congreso; OOMYCETE MOLECULAR GENETICS NETWORK MEETING 2011; 2011

OOMYCETE MOLECULAR GENETICS NETWORK

A partially open question is how microbial pathogens manipulate the plant immune system to establish disease. Oomycete effector proteins have been reported to be delivered to the host cell to alter host immunity and therefore allowing successful pathogen colonization. The Hyaloperonospora arabidopsidis genome potentially encodes approximately 140 candidate effector proteins likely involved in the alteration of plant defense processes to allow successful growth and reproduction. Using a high throughput yeast two hybrid technique a plant immune interactome was generated using 99 RxLRmotif containing effectors proteins against a collection of more than 8000 Arabidopsis proteins. The results made it possible to generate an interaction network and interesting candidates have been investigated further using fluorescence microscopy, Arabidopsis TDNA knock-out lines and RXLR over expression lines to deduce the effect of these proteins on plant susceptibility.