C-type lectins on macrophages participate in the immunomodulatory response to Fasciola hepatica products

GUASCONI, L; SERRADELL, MC; GARRO, AP; IACOBELLI, L; MASIH, DT

WILEY-BLACKWELL PUBLISHING, INC

Año: 2011 vol. 133 p. 386 - 386

asciola hepatica releases excretory-secretory products (FhESP), and immunomodulatory properties have been described for carbohydrates present in these parasite products. The interaction of FhESP with the innate immune cells, such as macrophages is crucial in the early stage of the infection. In this work we observed that peritoneal macrophages from naive Balb/c mice stimulated in vitro with FhESP presented: an increased arginase activity as well as Arg I expression, and high levels of TGF-β and IL-10. A similar macrophage population was also observed in the peritoneum of infected mice. A partial inhibition of the immunomodulatory effects described above was observed when macrophages were pre-incubated with Mannan, anti-Mannose receptor, Laminarin or anti-Dectin-1, and then stimulated with FhESP. In addition, we observed a partial inhibition of these effects in macrophages obtained from mice that were intraperitoneally injected with Mannan or Laminarin before being infected. Tak