Dectin-1 on macrophages modulates the immune response to Fasciola hepatica products through the ERK signaling pathway

GUASCONI, LORENA; BURSTEIN, VERÓNICA L.; BECCACECE, IGNACIO; MENA, CRISTIAN; CHIAPELLO, LAURA S.; MASIH, DIANA TERESA

IMMUNOBIOLOGY.

ELSEVIER GMBH

Año: 2018 vol. 223 p. 834 - 834

0171-2985

asciolosis is a zoonotic disease of increasing importance due to its worldwide distribution and elevated economic losses. Previously, we demonstrated that Fasciola hepatica excretory?secretory products (FhESP) induce immunomodulatory effects on peritoneal macrophages in a Dectin-1 dependent manner. In this study, we observed that peritoneal macrophages from naive BALB/c mice stimulated in vitro with FhESP presented increased expression levels of phosphorylated extracellular-signal-regulated kinase (ERK), and this effect was dependent on Syk, protein kinase C (PKC) and Dectin-1. In this sense, we observed increased levels of arginase activity, IL-10 and TGF-β in macrophages stimulated with FhESP, which were dependent on PKC and ERK. Furthermore, we observed that the increased arginase activity, as well as in TGF-β and IL-10 levels, was partially dependent on IL-10 receptor signaling in macrophages that were pre-incubated with anti-IL10R before being stimulated with FhESP. Tak