Immunomodulatory effects of Fasciola hepatica excretory-secretory products on macrophages

GUASCONI L; SERRADELL MC; MASIH DT; CHIAPELLO LS; CANCELA M; MAGGIOLI G

Fasciola hepatica Methods and Protocols

Springer

Año: 2020; p. 12 - 240

The liver fluke, Fasciola hepatica, infects a wide range of mammals including humans and leads to chronicdisease. Like other helminths, F. hepatica migrates and survives in the host tissues after penetrating theintestinal wall to enter the peritoneal cavity, and then migrates through the liver before finally inhabiting thebile ducts. To avoid the antihelminthic immune response during migration, F. hepatica releases excretory?secretory products (FhESP) that exert various immunomodulatory effects, such as alternative macrophageactivation or programmed cell death induction. Here, we describe the currently available techniquesfor studying macrophage activation and apoptotic cell death triggered by purified FhESP originating fromthe adult F. hepatica fluke.