MASS SPECTROMETRY STUDIES OF LYCOPODIUM ALKALOIDS LYCODINE-TYPE: SAUROXINE AND N-DEMETHYL-SAUROXINE

D.A. CIFUENTE; M.G. VALLEJO; M.G. ORTEGA; J.L. CABRERA; V.S. MARTIN; C.E. TONN; A.M. AGNESE; C. ARDANAZ

RAPID COMMUNICATIONS IN MASS SPECTROMETRY : RCM.

JOHN WILEY & SONS LTD

Lugar: LOndres; Año: 2014 vol. 28 p. 2690 - 2690

0951-4198

ATIONALE: Sauroxine and N-Demethyl-sauroxine are Lycopodiumalkaloids lycodine-type. During the last years Lycopodium alkaloids havegained a significant interest due to their unique skeletal characteristics aswell as due to their acetylcholinesterase activity. Is known that drugs thatinhibit acetylcholinesterase, can be used to treat early stages of Alzheimerdisease.METHODS: Sauroxine and N-Demethyl-sauroxine were isolated from aerialparts of Huperzia saururus (Lam.) Trevis. Electron ionization massspectrometry (EI-MS) (Low Resolution) and collision-induced dissociationtandem mass spectrometry in time (CID-MS/MS) fragmentation wasconducted by an ion trap, GCQ Plus mass spectrometry with MS/MS. EIhigh-resolution mass spectrometry (EI-HRMS) was performed in amagnetic sector mass spectrometer Micromass VG.RESULTS: Using GC/EI-CID-MS/MS we obtained the differentfragmentation routes that connect all the ioni