A NEW SEMI-SYNTHETIC DERIVATIVE OF SAUROINE INDUCES LTP IN HIPPOCAMPAL SLICES AND IMPROVES LEARNING PERFORMANCE IN THE MORRIS WATER MAZE

M.G. VALLEJO; S. LOYOLA; D. CONTRERAS; G. UGARTE; D.A. CIFUENTE; M.G. ORTEGA; J.L. CABRERA; M. ZEISE; C.E. TONN; M. CARREÑO; R. DELGADO; B. MORALES; A. M. AGNESE

JOURNAL OF NEUROCHEMISTRY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2014 p. 864 - 864

0022-3042

wo semi-synthetic acetyl-derivatives of the alkaloid sauroine fromHuperzia saururus were obtained: Monoacetyl sauroine (MAS) and diacetylsauroine (DAS), and their chemical structures analyzed by NMR. While MAS isthe typical product of acetylation, DAS is an unexpected derivative related to theketo-enol formation of sauroine. Recordings of field EPSPs from the CA1 region of rat hippocampal slices showed that only DAS acutely applied induced chemical Long-Term Potentiation (LTP) in a dose-dependent manner with an EC50 of 1.15±0.09 μM. This effectwas blocked by 10 μM D(-)-2-amino-5-phosphonopentanoic acid (AP5), suggesting dependence on the NMDA receptor. DAS significantly increased NMDA receptor-dependent EPSCs without affecting AMPA receptor-dependent currents. Repetitive administration of DAS improved visuo-spatial learning in the Morris Water Maze (MWM). In slices from rats tested in the MWM, LTP resulting from electrical synaptic stimulation w