Albumin biofunctionalization to minimize the Staphylococcus aureus adhesion on solid substrates

MARTÍN, MARÍA LAURA; PFAFFEN, VALERIA; VALENTI, LAURA E.; GIACOMELLI, CARLA E.

COLLOIDS AND SURFACES B-BIOINTERFACES

ELSEVIER SCIENCE BV

Año: 2018 vol. 167 p. 156 - 156

0927-7765

Staphylococcus aureus has become the most common opportunistic microorganism related to nosocomialinfections due to the bacteria capacity to form biofilms on biomedical devices and implants. Since bacte-rial adhesion is the first step in this pathogenesis, it is evident that inhibiting such a process will reducethe opportunity for bacterial colonization on the devices. This work is aimed at optimizing a surfacebiofunctionalization strategy to inhibit the adhesion of S. aureus on solid substrates. The first part of thework deals with the albumin adsorption-desorption process, studied by a factorial design of experimentsto explore a wide range of experimental factors (protein concentration, pH, flow rate and adsorptiontime) and responses (initial adsorption rate, adsorbed amount, desorbed extent) for hydrophilic andhydrophobic substrates, with a reduced number of experiments. This approach allows the simultaneousevaluation of the factors affecting the albumin adsorption-desorption proce