A simple surface biofunctionalization strategy to inhibit the biofilm formation by Staphylococcus aureus on solid substrates

MARTÍN, MARÍA LAURA; DASSIE, SERGIO A.; VALENTI, LAURA E.; GIACOMELLI, CARLA E.

COLLOIDS AND SURFACES B-BIOINTERFACES

ELSEVIER SCIENCE BV

Año: 2019 vol. 183

0927-7765

taphylococcus aureus is an important opportunistic pathogen that causes a broad range of infections due to the bacteria capacity to form biofilms on medical devices. This work is aimed at inhibiting the biofilm formation by S. aureus on solid substrates using a simple surface biofunctionalization strategy. We previously found that surface biofunctionalization with structural perturbed albumin inhibited the initial stage of S. aureus adhesion. The current work extends this strategy with other plasma protein, fibrinogen, which in addition can be bond specifically to the cell wall?anchored proteins of S. aureus. The study of fibrinogen adsorption indicates that the fraction of surface-perturbed molecules is enlarged at long adsorption times and low protein concentration. In these conditions, a significant diminution of ca.60% of alive adhered bacteria were observed after 40 min and the biofilm formation was completely prevented. Thus, it seems that the inhibition of bacterial adhesion on