HPLC Method for the Determination of Nystatin in Saliva for Application in Clinical Studies

MANUEL LLABOT, JUAN; DANIEL ALBERTO ALLEMANDI; MARCELA LONGHI,

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Elsevier

Año: 2007 vol. 25 p. 526 - 526

0731-7085

An isocratic high-performance liquid chromatographic method was developed, optimized and validated for the determination of nystatin in human saliva (UV and fluorescence detection). A reversed-phase LunaTM C18 column (25 ◦C), with a mobile phase of MeOH, H2O, and DMF(70:20:10, v/v/v), and a flow-rate of 0.8 ml/min were used. The elution time for nystatin was 5.8±0.2 min. Calibration curves in human saliva were linear from 0.78 to 50 g/ml. Limits of quantification were 0.78 g/ml and 0.75 g/ml for UV and fluorescence detection, respectively. The accuracy and precision values of intra- and inter-day variation studies were within acceptable limits, according to FDA guidelines. The described method has proved to be useful to give accurate measurements of nystatin in real samples.