Phase behavior of ascorbyl palmitate coagels loaded with oligonucleotides as a new carrier for vaccine adjuvants

GABRIELA ULLIO GAMBOA; LUCIANO BENEDINI; PABLO C. SCHULZ; DANIEL ALLEMANDI

JOURNAL OF SURFACTANTS AND DETERGENTS

Springer US

Lugar: New York; Año: 2016 vol. 19 p. 747 - 747

1097-3958

n this work, the phase behavior variations of an ascorbyl palmitate (Asc16) system in aqueous solution were analyzed when immunologically active hydrophiliccompounds (CpG and OVA) were introduced. This study was carried out through optical polarizing microscopy (OPM) and differential scanning calorimetry (DSC) at different temperatures and over a broad range of concentrations. The combination of both techniques allowed the determination of a complete phase diagram which was compared with those built for Asc16-water system and itwas demonstrated that fixed concentrations of hydrophilic compounds (300 and 24 lg/g for CpG-ODN and OVA respectively) generate two lamellar liquid crystals, a cubic liquid crystal phase, and also other aggregates. However, no changes were observed in the phase diagram in terms of formation of new mesophases. The aqueous phase behavior was also studied as a function of surfactant and temperature. DSC and Fourier transform infrared spectroscopy (FT-IR) measureme