A liquid crystal of ascorbyl palmitate, used as vaccine platform, provides sustained release of antigen and has intrinsic pro-inflammatory and adjuvant activities which are dependent on MyD88 adaptor protein.

MARÍA F. SÁNCHEZ VALLECILLO; MARÍA M. MINGUITO DE LA ESCALERA; MARÍA V. AGUIRRE; GABRIELA V. ULLIO GAMBOA; SANTIAGO D. PALMA; LETICIA GONZÁLEZ-CINTADO; ANA L. CHIODETTI; GERMÁN SOLDANO; GABRIEL MORÓN; DANIEL A. ALLEMANDI; CARLOS ARDAVÍN; MARÍA C. PISTORESI-PALENCIA; BELKYS A. MALETTO

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015

odern subunit vaccines require the development of new adjuvant strategies. Recently,we showed that CpG-ODN formulated with a liquid crystal nanostructure formed by self-assembly of 6-O-ascorbyl palmitate (Coa-ASC16) is an attractive system for promoting an antigen-specific immune response to weak antigens. Here, we showed that after subcutaneous injection of mice with near-infrared fluorescent dye-labeled OVA antigen formulated with Coa-ASC16, the dye-OVA was retained at the injection site for a longer period than when soluble dye-OVA was administered. Coa-ASC16 alone elicited a local inflammation, but how this material triggers this response has not been described yet. Although it is known that some materials used as a platform are not immunologically inert, very few studies have directly focused on this topic. In this study, we explored the underlying mechanisms concerning the interaction between Coa-A