Class-B CpG-ODN formulated with a nanostructure induces type I interferons-dependent and CD4+T cell-independent CD8+T-Cell response against unconjugated protein antigen

CHIODETTI, ANA L.; SÁNCHEZ VALLECILLO, MARÍA F.; DOLINA, JOSEPH S.; CRESPO, MARÍA I.; MARIN, CONSTANZA; SCHOENBERGER, STEPHEN P.; ALLEMANDI, DANIEL A.; PALMA, SANTIAGO D.; PISTORESI-PALENCIA, MARÍA C.; MORÓN, GABRIEL; MALETTO, BELKYS A.

Frontiers in Immunology

Frontiers Media S.A.

Año: 2018 vol. 9

here is a need for new vaccine adjuvant strategies that offer both vigorous antibody and T-cell mediated protection to combat difficult intracellular pathogens and cancer. To this aim, we formulated class-B synthetic oligodeoxynucleotide containing unmethylated cytosine-guanine motifs (CpG-ODN) with a nanostructure (Coa-ASC16 or coagel) formed by self-assembly of 6-0-ascorbyl palmitate ester. Our previous results demonstrated that mice immunized with ovalbumin (OVA) and CpG-ODN formulated with Coa-ASC16 (OVA/CpG-ODN/Coa-ASC16) elicited strong antibodies (IgG1 and IgG2a) and Th1/Th17 cellular responses without toxic systemic effects. These responses were superior to those induced by a solution of OVA with CpG-ODN or OVA/CpG-ODN formulated with aluminum salts. In this study, we investigated the capacity of this adjuvant strategy (CpG-ODN/Coa-ASC16) to elicit CD8+ T-cell response and some of the underlying cellular and molecular mechanisms involved in adaptive response. We also analyzed