Protective role of melatonin on retinal ganglionar cell: In vitro an in vivo evidences

DEL VALLE BESSONE, CAROLINA; FAJRELDINES, HUGO DIAZ; DE BARBOZA, GABRIELA EDIT DIAZ; DE TALAMONI, NORI GRACIELA TOLOSA; ALLEMANDI, DANIEL ALBERTO; CARPENTIERI, AGATA RITA; QUINTEROS, DANIELA ALEJANDRA

LIFE SCIENCES

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2019 vol. 218 p. 233 - 233

0024-3205

xidative stress triggers ocular neurodegenerative diseases, such as glaucoma or macular degeneration. The increase of reactive oxygen and nitrogen species in retinal ganglion cells (RGCs) causes damage to the structure and function of the axons that make up the optic nerve, leading to cell death arising from apoptosis, necrosis or autophagy in the RCGs. The use of antioxidants to prevent visual neurodegenerative pathologies is a novel and possibly valuable therapeutic strategy. To investigate in vitro and in vivo neuroprotective efficacy of melatonin (MEL) in RGCs, we used a model of oxidative glutamate (GLUT) toxicity in combination with L-butionin-S, R-sulfoximine (BSO), which induces cell death by apoptosis through cytotoxicity and oxidative stress mechanisms. Histological sectioning and immunohistochemical assays using the TUNEL technique were performed to determine the damage generated in affected cells and to observe the death process of RGCs. Whit BSO-GLUT the results revealed