Spray dried microspheres based on chitosan: a promising new carrier for intranasal administration of polymeric antigen BLSOmp31 for prevention of ovine brucellosis.

DÍAZ AG, ; QUINTEROS DA, ; LLABOT JM, ; PALMA SD, ; ALLEMANDI DA, ; GHERSI G, ; ZYLBERMAN V, ; GOLDBAUM FA, ; ESTEIN SM.

MATERIALS SCIENCE & ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 63 p. 489 - 489

0928-4931

revious studies have demonstrated that parenteral immunization with polymeric antigen BLSOmp31 induced a strong immune response and conferred protection against Brucella ovis in rams. This work describes the development of a novel formulation strategy for the delivery of BLSOmp31 in the nasal mucosa. Chitosan microparticles were prepared by spray-drying technology processes and recombinant chimera BLSOmp31 was loaded by passive adsorption onto chitosan microspheres, which were characterized by means of the evaluation of size, zetapotential, morphology, and loading and release rate of BLSOmp31. The mucoadhesive properties of microspheres were evaluated by studying the interaction between microparticles and mucin. The antigen BLSOmp31 integritywas investigated by SDS-PAGE. The yield of production of spray-drying process was 68.95%. Microspheres had a good sphericity, 1?10 μm of particle size and had a positive charge. The loading capacity was found to be 45.19%.The initial fast rel