ENHANCEMENT OF IMMUNOLOGICAL ACTIVITY OF CPG-ODN VEHICULIZED IN LYOTROPIC LIQUID CRYSTAL CARRIERS

MALETTO B.; ALLEMANDI D.A.; MORÓN G.; PISTORESI- PALENCIA M.C.; PALMA S.D.

Tandil

Congreso; XL Reunión de la Sociedad Argentina de Farmacología Experimental; 2008

CpG-ODN (oligonucleotides containing unmethylated CpG in particular base context) is a new adjuvant for vaccines that unfortunately has shown reduced bioavailability owed mainly to lack of chemical stability. In this work, we evaluated the adjuvant capacity of CpG-ODN incorporated into lamellar mesophases of lyotropic liquid crystals (coagels). For that, BALB/c mices were immunized (s.c.) three times during fifteen days (schedule: 0, 7 and 15) with a protein antigen (OVA, 60 ug/mice/dose) plus CpG-ODN (50ug/mice/dose)or OVA/CpGODN/ coagel. On day 21, we measured specific antibodies in plasma and specific cytokines in cell culture supernatants (ELISA)