Asparagine quantification in cellular culture media using copper modified carbon nanotubes composite electrodes

M. L. LÓPEZ RODRIGUEZ, G. L. LUQUE, C. J. FELICE, N. F. FERREYRA, R. E. MADRID, G A. RIVAS, C. E. GIACOMELLI

ELSEVIER SCIENCE SA

Año: 2011 vol. 158 p. 423 - 423

Direct quantification of Asparagine (Asp) in a Streptomyces sp. M7 (SM7) culture medium was performed using multi-wall carbon nanotubes composite electrodes modified with copper microparticles (CNTPE-Cu). A sensitive and fast response is achieved at CNTPE-Cu after additions of SM7 cultures supernatant, without interference of the culture medium components. This response allows determining the Asp concentration as a function of the bacteria growth. The results reveal that the SM7 bacteria consume Asp as a carbon and nitrogen sources in the absence of another